FIG 2.

Deletion of ycaL improves the viability and LptD levels of a lptDY721D strain. (A) Antibiotic sensitivity was assessed by spotting serial 10-fold dilutions of stationary-phase cultures of strains containing the indicated mutations on LB agar, with or without vancomycin or bacitracin, and incubating the cultures at 37°C. (B) Relative LptD levels were determined in a wild-type strain and strains containing each of the chromosomal mutations listed, in a lptDY721D background, by SDS-PAGE and immunoblotting for the proteins indicated. (C) CFU were determined in control and lptDY721D strains containing each of the mutations listed by transformation with the indicated plasmids, i.e., pZS21 (−), pZS21::bamD (+), or pZS21::bamD O/E (++), and calculation of the resulting surviving colonies. (D) Relative protein levels were determined from whole-cell lysates from a wild-type strain and strains containing each of the chromosomal mutations and plasmids listed, in a lptDY721D background, by SDS-PAGE and immunoblotting for the proteins indicated. Red, reduced; Ox, oxidized.