TABLE 1.
Outer membrane permeability and LptD assembly phenotypes of constructed protease mutants
| Genotype | Wild-type background |
lptD4213 background |
lptE6 background |
lptDY721D background |
||||
|---|---|---|---|---|---|---|---|---|
| OM phenotypea | LptD restorationb | OM phenotype | LptD restoration | OM phenotype | LptD restoration | OM phenotype | LptD restoration | |
| MC4100 | ++++ | NA | ++ | − | ++ | − | ++ | − |
| degP::kan | ++++ | NA | + | − | ++ | − | + | +++ |
| degQ::kan | ++++ | NA | ++ | − | ++ | − | ++ | − |
| ompT::kan | ++++ | NA | ++ | − | ++ | − | ++ | − |
| ptrA::kan | ++++ | NA | ++ | − | ++ | − | ++ | − |
| tsp::kan | ++++ | NA | ++ | − | +++ | − | ++ | − |
| ycaL::kan | ++++ | NA | ++ | − | ++ | − | +++ | ++ |
| ydgD::kan | ++++ | NA | ++ | − | ++ | − | ++ | − |
| loiP::kan | ++++ | NA | ++ | − | ++ | − | ++ | − |
| bepA::kan | ++ | NA | + | +++ | ++ | ++ | ++ | − |
| yhiJ::kan | ++++ | NA | ++ | − | ++ | − | ++ | − |
a
OM phenotype: ++++, growth similar to wild-type growth in the presence of antibiotics; −, no growth.
b
LptD restoration: NA, not applicable; ++++, restoration of LptD levels close to wild-type levels; −, no restoration of LptD levels.