FIG 2.

PccD (SACE_3396) binds to the promoter regions of pccBC and pccA. (A) Genetic organization of the pccD gene in the S. erythraea genome. pccB (SACE_3398), pccC (SACE_3399), and pccA (SACE_3400) encode a propionyl-CoA carboxylase β-chain, acetyl-CoA carboxylase probable ε-subunit, and acetyl/propionyl-CoA carboxylase α-subunit, respectively. The numbers between dashed lines show the lengths of the promoter regions of pccBC and pccA, respectively, used in the gel shift experiment shown in panel B. (B) EMSA of His-PccD protein with promoter regions (separated by dashed lines shown in panel A) of pccBC or pccA. The biotin-labeled DNA probe (about 15 ng, reaction system 10 μl) was incubated with a protein concentration gradient (0, 1, 3, and 5 μM). An unlabeled specific probe (200-fold) or nonspecific competitor DNA (200-fold, sonicated salmon sperm DNA) was used as control. The free probes that did not bind with protein are shown by arrowheads.