FIG 3.

PccD is a repressor of pccBC and pccA operons. (A) qPCR analysis of the relative transcription levels of pccD, pccBC, and pccA genes between the WT, ΔpccD, and WT/PIB-pccD strains. Total RNA of these strains was extracted after 36 h of growth in TSB medium (see Fig. S5A in the supplemental material). The expression levels of genes in the WT strain were arbitrarily set to 1.0. Error bars indicate standard deviation from three independent experiments. (B and C) Growth curves of S. erythraea strains WT, ΔpccD, and WT/PIB-pccD grown on Evans medium supplemented with 20 mM sodium propionate (B) or 2.5% glucose (C) as the sole carbon source. Error bars indicate standard deviation from three biological replicates.