FIG 2.

ChIP-seq analysis of in vivo binding sites of AphB on V. alginolyticus chromosomes. (A) The function of Flag-tagged AphB (AphB-Flag) remained intact in V. alginolyticus. AphB-Flag or AphB expression driven by pBAD was induced by l-arabinose (Ara), and extracellular Asp production was assayed in the indicated strains with (+) or without (−) the addition of Ara. **, P < 0.01, Student's t test, relative to results for the absence of arabinose. (B) The significant motif that resulted from the ChIP-seq binding sequences was identified. The height of each letter represents the relative frequency of each base at each position in the consensus sequence. The count matrix for the binding sequences is also shown.