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(a) Normalized fluorescence emission spectra of HSA (15.04×10–6 mol L−1) in the presence of increasing concentrations of EA. EA/HSA corresponds to 0, 0.15, 0.31, 0.47, 0.62, 0.78, 0.94, 1.09 and 1.41 respectively. pH=7.4, T=298 K, λex=295 nm, λem=339 nm (b) Stern-Volmer plot for the quenching of HSA by EA, at 298 K. (c) Modified Stern-Volmer plot of HSA-EA system.
