Table 4.
Comparison among three different protocols to obtain total genomic DNA using NanoDrop
| Species | Mondragón-Jacobo et al. [11] | Griffith and Porter [13] | This protocol | |||
|---|---|---|---|---|---|---|
| DNA yield (ng/µl) | OD ratio 260.280 | DNA yield (ng/µl) | OD ratio 260.280 | DNA yield (ng/µl) | OD ratio 260.280 | |
| 1. Opuntia auberi Pfeiff. | 256 | 1.4 | 423 | 1.7 | 1600 | 1.9 |
| 2. Opuntia decumbens Salm-Dyck | 35 | 1.7 | 30 | 1.9 | 2930 | 1.9 |
| 3. Opuntia delafuentiana Martínez-González et al. | 75 | 1.6 | 56 | 1.9 | 4937 | 1.8 |
| 4. Opuntia depressa Britton and Tose | 95 | 1.9 | 73 | 1.8 | 8755 | 1.9 |
| 5. Opuntia durangensis Britton and Tose | 134 | 1.5 | 123 | 1.7 | 5835 | 1.9 |
| 6. Opuntia ficus-indica Mill. | 34 | 1.8 | 258 | 1.8 | 3829 | 2.0 |
| 7. Opuntia heliabravoana Scheinvar | 198 | 1.6 | 43 | 1.8 | 8743 | 1.8 |
| 8. Opuntia huajuapensis Bravo | 57 | 1.5 | 78 | 1.7 | 1573 | 1.9 |
| 9. Opuntia joconostle F.A.C. Weber | 86 | 1.9 | 196 | 1.0 | 8375 | 1.8 |
| 10. Opuntia lasiacantha Pfeiff. | 67 | 1.7 | 356 | 1.7 | 2943 | 1.9 |
| 11. Opuntia leiascheinvariana Martínez-González | 110 | 1.8 | 98 | 1.9 | 3980 | 1.9 |
| 12. Opuntia leucotricha DC. | 248 | 1.5 | 34 | 1.8 | 3789 | 1.9 |
| 13. Opuntia matudae Scheinvar | 93 | 1.7 | 63 | 1.8 | 7947 | 1.9 |
| 14. Opuntia megacantha Salm-Dyck | 117 | 1.6 | 78 | 1.8 | 7000 | 1.8 |
| 15. Opuntia microdasys Pfeiff. | 44 | 1.5 | 39 | 1.7 | 6578 | 1.9 |
| 16. Opuntia oligacantha Förster | 87 | 1.8 | 70 | 1.9 | 2395 | 1.8 |
| 17. Opuntia olmeca Joel Pérez et al. | 94 | 1.5 | 57 | 1.7 | 9200 | 1.9 |