Table 5.

Advantages of our protocol

Mondragón-Jacobo et al. [11]	Griffith and Porter [13]	This protocol
They tried to use young tissues, avoiding older ones because their higher content of fiber and cuticular wax	They tried to use epidermal tissue free of waxes	We can use tissue from any part of the plant
They used β-mercaptoethanol	They used β-mercaptoethanol	We did not use β-mercaptoethanol
8000 mg of cactus pear tissue	30–50 mg of epidermal tissue	2–3 mg of tissue from every part of the plant
They used more CTAB (25 ml)	They used more CTAB (15 ml)	We used few CTAB (0.7 ml)
They used more chloroform-isoamyl alcohol (10 ml)	They used more chloroform-isoamyl alcohol (5 ml)	We used few chloroform-isoamyl alcohol (0.75 ml)
They used ethanol (8.7 ml)	They used more isopropanol (5 ml)	We used few isopropanol (0.4 ml)
They used bigger and expensive tubes (15 ml)	They used bigger and expensive tubes (15 ml)	We used smaller tubes (2 ml)
They used RNAse to eliminate RNA	They did not use RNAse	We did not use RNAse