Table 2.

Intra and inter-run accuracy and precision of the method (n = 5)

Concentration (ng/mL)		Intra-run		Inter-run
		Accuracy	R.S.D. (%)	Accuracy	R.S.D. (%)
Plasma
Nicotine
LLOQ	3	101.49	7.50	107.06	9.53
LQC	9	93.36	5.02	98.92	9.33
MQC	75	89.67	3.55	89.35	4.64
HQC	150	92.48	5.90	98.61	11.30
Cotinine
LLOQ	3	84.34	3.7	94.29	10.99
LQC	9	105.98	6.93	100.25	14.40
MQC	225	90.55	1.24	89.96	6.97
HQC	450	97.89	9.5	98.31	8.13
Brain
Nicotine
LLOQ	3	104.26	4.36	102.59	14.48
LQC	9	111.01	4.93	107.28	5.65
MQC	40	114.45	7.36	109.44	9.10
HQC	80	113.46	6.91	110.70	7.51
Cotinine
LLOQ	3	96.66	8.59	98.55	8.01
LQC	9	107.36	6.56	106.23	5.46
MQC	40	106.64	9.68	109.61	8.20
HQC	80	112.52	1.26	113.21	1.49