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Conventional pH optimum determination of Celluclast® at three temperatures. The pH optimum of Celluclast® on barley-β-glucan was determined at 45 °C, 55 °C, and 65 °C. At lower temperatures, the enzyme can tolerate higher pH values. This is in accordance with the corresponding contour plot and shows the strong impact of the chosen fixed parameter when determining the pH or temperature optimum separately
