Table 1.

Overview of the different enzymes, substrates, and assay conditions

Enzyme	Assay	Substrate	Substrate conc. [mg/ml]	Enzyme conc. [µg/ml]	Assay duration [min]	Temp. gradient [°C]	pH gradient
Cel8A	DNSA	BBG	3.3	0.5	90	60.1 ± 19.9	4.0–8.0
	Azo-CMC	Azo-CMC	3.3	5	90	60.1 ± 19.9	4.0–8.0
Celluclast®	DNSA	BBG	3.3	5	90	55.0 ± 19.9	4.0–8.0
		AX	3.3	200	90	55.0 ± 19.9	4.0–8.0
	p-NP	p-NP-β-d-gluco-pyranoside	1.9 mM	50	15	55.0 ± 19.9	4.0–8.0
	Glucose HK	Wheat straw	10	250	90	55.0 ± 19.9	4.0–8.0