Table 2.
Mutations in pncA and the proteins of the special set selected and the best candidates from the global genome analysis by mutation that appeared to be statistically significant
| PZAse (Rv2043c) | Metal Ion transporters | Efflux pumps | Reported proteins | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Isolate | Res PZA | Gene | Protein | Promoter | % of PZAse activity b | NicT | Ctpl | CtpE | Rv0194 | MppL11 | MmpL3 | MgtE | Rv0987 | MmpL13a | MmpL10 | Rv1348 | Rv3239c | Mez | Rv2994 | RpsA | GpsI | PstC2 |
| CSV4519 | 1 | – | – | – | 100.00 | – | – | – | A968V | – | – | – | – | – | – | – | P548S | – | – | – | – | – |
| CSV4644 | 1 | T152C | H51R | – | 0.02 | Y357C | – | – | – | – | E317Q | – | – | L830 V | – | – | A381V | – | – | |||
| CSV5769 | 1 | C232A | G78C | – | 18.12 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| CSV10399 | 1 | A280G | F94 L | – | 55.18 | – | – | – | – | – | – | A77S | V83F | – | – | – | – | – | – | – | – | |
| CSV11678 | 1 | G185A | P62L | – | NA | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| LE486 | 1 | T35G | D12A | – | 24.06 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| LE492 | 1 | T35G | D12A | – | 24.06 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| LN180 | 1 | A392C | V131G | – | NA | – | – | – | – | – | – | – | W797a | – | – | – | – | – | – | – | – | |
| LN2358 | 1 | A545C | L182 W | – | NA | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| LN3756 | 1 | T403G | T135P | – | 0.05 | – | N355 K | – | – | – | – | – | – | – | L218 V | – | – | – | – | P96R | – | |
| MDRDM260 | 1 | T403G | T135P | – | 0.05 | – | N355 K | – | – | – | – | – | – | – | L218 V | – | – | – | – | P96R | – | |
| MDRDM627 | 1 | – | – | – | 100.00 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | |
| MDRDM1098 | 1 | C145T | D49N | – | 0.12 | – | A1469P | – | A302T | – | E907A | – | – | – | – | – | – | – | M432 T | – | – | |
| MDRMA2491 | 1 | T35C | D12G | – | 36.45 | – | – | – | – | – | – | S265G | – | – | – | – | – | – | ||||
| ME1473 | 1 | A100C | Y34D | – | 53.59 | – | – | – | – | C263R | – | – | T142A | – | – | – | – | W214a | – | – | ||
| TBDM425 | 1 | C145T | D49N | – | 0.12 | – | A1469P | – | A302T | – | E907A | – | – | – | – | – | – | – | M432 T | – | – | |
| TBV5000 | 1 | – | – | – | 100.00 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | ||
| TBV5362 | 1 | – | – | – | 100.00 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | ||
| TBV5365 | 1 | – | – | – | 100.00 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SLM036 | 1 | A190C | Y64D | – | NA | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SLM040 | 1 | T170C | H57R | – | NA | – | – | – | – | – | – | – | – | – | – | – | – | D46E | – | – | – | W162G |
| SLM056 | 1 | – | – | – | 100.00 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SLM060 | 1 | – | – | T-11C | c | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | ||
| SLM063 | 1 | A416G | V139A | – | NA | – | – | A653E | – | – | – | – | W797a | – | – | – | – | – | – | – | – | – |
| SLM088 | 1 | – | – | – | NA | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SLM100 | 1 | – | – | T-11C | c | – | M920I | – | – | – | – | – | – | – | – | – | V904I | – | – | – | – | – |
We are only showing the mutations that were only present in PZA-resistant strains. Strains harboring mutations strongly related with PZA resistance and strains with no mutations in PZA, neither in the specific set of genes selected, are in bold
aAminoacid deletions
bWild-PZAse (H37Rv) activity = 100%. NA: Not available
cAffects the expression of the Pyrazinamidase, but not its enzimatic activity