Extended Data Figure 2.
Interference from the reporter plasmid. a) Oscillations of the integrated repressilator with the PLtet-mCherry-asv plasmid (LPT54) have a more constant peak amplitude compared to the original repressilator, indicated by the CV of the peak amplitude decreasing from 0.78 to 0.36 (b). The inset in b) zooms in on the tails of the distributions. c) Additional plasmid loss event of integrated repressilator with PLtet-mCherry-asv reporter. The reporter plasmid is lost around generation 34, as evidenced by the loss of red fluorescence. The oscillation period shifts from ~2 to ~5 generations quickly after the plasmid loss event. d) Example time trace of the integrated repressilator, without the reporter plasmid (LPT25). The YFP production rate oscillates (yellow trace), while the segmentation marker (blue trace) stays relatively constant (close-up of the shaded region on top). Both traces were normalized to their respective means. e) Autocorrelation function (ACF) and power spectral density (PSD) were calculated over the whole population (8,694 total generations) and demonstrate strong oscillatory behavior, with an average period of 5.6 generations. The width of the window function used for calculating the power spectrum is indicated by a red line.