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. Author manuscript; available in PMC: 2017 Oct 12.
Published in final edited form as: J Hum Genet. 2017 Feb 23;62(6):589–597. doi: 10.1038/jhg.2017.19

Figure 2.

Figure 2

GluN1-G620R changes agonist potency. (a and b) Representative two-electrode voltage-clamp recordings obtained from oocytes expressing wild-type GluN1/GluN2A receptors in which the currents were evoked by increasing concentrations (μM) of glutamate (a, in the presence of 100 μM glycine) and glycine (b, in the presence of 100 μM glutamate) at the holding potential of − 40 mV. (c–f) Composite concentration-response curves determined by two-electrode voltage-clamp (TEVC) recordings from Xenopus oocytes are shown for wild-type GluN1 or GluN1-G620R co-expressed with GluN2A (c and d) or GluN2B (e and f). Glutamate (c and e) and glycine (d and f) concentration-effect curves showed that the GluN1-G620R mutant has a reduced agonist potency (increased EC50 values, see Table 1) for both agonists.