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. Author manuscript; available in PMC: 2017 Oct 12.
Published in final edited form as: Cell Rep. 2017 Oct 10;21(2):495–507. doi: 10.1016/j.celrep.2017.09.057

Figure 4. Brachyury directly activates transcription of YAP.

Figure 4

a, Brachyury binding to the proximal promoter region of YAP as observed in a publicly available ChIP-sequencing dataset using UCH1 cells. b, ChIP-PCR querying across the −1.5 kb region upstream of the transcriptional start site (TSS) of the YAP promoter in JHC7 cells using two independent antibodies specific for epitopes on the C- (Ab1) and N-terminus (Ab2) of brachyury. c, Representative immunoblot of brachyury and YAP expression in siCtrl or siT JHC7 cells. d, YAP gene promoter luciferase assay using the −105bp and −608bp regions upstream of the TSS in siCtrl or siT JHC7 cells. e, Schematic of transcriptional regulation of YAP by brachyury. All error bars are s.e.m. * = P<0.05. See also Figure S5.