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. Author manuscript; available in PMC: 2017 Oct 12.
Published in final edited form as: Cell Rep. 2017 Oct 10;21(2):495–507. doi: 10.1016/j.celrep.2017.09.057

Figure 5. Brachyury regulates stemness and proliferation through YAP.

Figure 5

a, Average percentage of cells in each cell cycle phase in shCtrl or shYAP JHC7 cells. b, Representative in vitro extreme limiting dilution assay plating decreasing numbers of shCtrl or shYAP JHC7 cells. c, Representative long-term MTT proliferation assay of shCtrl or shYAP JHC7 cells. d, Representative immunoblot of YAP, Cyclin-D1, and Survivin expression in shCtrl or shT JHC7 cells transfected with empty vector (CONT) or YAP-overexpression (YAP-OE) plasmids. e, mRNA expression of cell cycle regulator genes in shCtrl or shT JHC7 cells with CONT or YAP-OE overexpression. f, Average percentage of cells in G1 phase in shCtrl or shYAP JHC7 cells with CONT or YAP-OE overexpression. g, Representative in vitro extreme limiting dilution assay plating decreasing numbers of shCtrl or shYAP JHC7 cells with CONT or YAP-OE overexpression. Solid lines: mean; dotted lines: 95% confidence interval; circles: values obtained in each cell dilution. h, Representative long-term MTT proliferation assay of shCtrl or shYAP JHC7 cells with CONT or YAP-OE overexpression. In h, * = significant versus Day 0, # = significant versus shCtrl CONT group for the same day. All error bars are s.e.m. *, # = P<0.05. See also Figure S6.