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. 2017 Jul 24;97(4):1049–1061. doi: 10.4269/ajtmh.17-0003

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Figure 1. — Identification of monoclonal antibodies (mAbs) against nonstructural protein 1 (NS1) by immunofluorescence assay, cellular enzyme-linked immunosorbent assay (ELISA), and Western blot analysis. (A) The indicated mAbs (DB6-1, DB12-3, DB16-1, DB20-6, DB29-1, DB38-1, and DB41-2) were individually incubated with BHK21 cells infected by dengue virus type 2 (DENV-2) (strain 16681). After secondary antibody and DAPI staining, the signals were detected by immunofluorescence microscopy. (B) C6/36 cells infected with the indicated serotype were incubated with the indicated mAbs for analysis by cellular ELISA. (C) Lysates from C6/36 cells infected with the indicated serotypes of DENV were separated on a reducing gel. Antigens of four DENV serotypes were recognized by DB16-1, DB20-6, and DB29-1. DENV2-specific NS1 protein was recognized by DB6-1, DB12-3, and DB38-1. DB41-2 can recognize NS1 proteins of DENV-2 and DENV-4. This figure appears in color at www.ajtmh.org.