Figure 4.

mtDNA transcript levels are higher in haplogroup M than in haplogroup N. Relative mtRNA expression was quantified by quantitative real-time PCR. The mRNA levels of 16S rRNA, ND1, and 7S RNA from the primary transcript represent H1, H2, and L strands of mtDNA, respectively. (a) Increased relative transcript level of mitochondrial L strand in haplogroup M. (b) Relative level of mitochondrial H1 strand transcript. (c) Higher relative transcript level of mitochondrial H2 strand in haplogroup M than in haplogroup N. Data represent mean ± SEM of two independent experiments with 3 replicates each per cybrid. ^∗∗∗P < 0.001.