Figure 5.

Immunofluorescent analysis of NF-κB nuclear translocation. (a) UVB irradiation induces NF-κB translocation into the nucleus. NF-κB (red) remained in the cytoplasm in naïve mice, while nano-AST (50 mg/kg) treatment markedly reduced translocalization (pink) of NF-κB in the nuclei (blue). Obtained immunohistochemistry data do not indicate significant signal change in AST oil-, lutein-, or bilberry extract-treated groups. Scale bar = 25 μm. (b) For analysis, mean values of NF-κB-positive cells were assessed. Numbers of NF-κB-positive cells were significantly reduced in the nano-AST group relative to other irradiated groups (p < 0.001). n = 8 (eyes) per group. n.s., p > 0.05; ^∗∗∗p < 0.001