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. 2017 Jul 31;23(10):790–797. doi: 10.1002/psc.3027

Figure 1.

Figure 1

Schematic overview of this research. (A) Disulfide‐constrained random peptide libraries (X3CX7–10CX3 random peptides, where X represents the randomized amino acid positions) constructed on T7 phage using phage display technology. (B) Three IgY binding phage clones (Y4‐4, Y5‐14, and Y5‐55) identified thorough repeated biopanning against IgY from random peptide libraries. (C) Biotinylated IgY‐binding peptides derived from Y4‐4 and Y5‐55 phage clones. (D) Affinity column conjugated with biotinylated Y4‐4 peptide on a HiTrap™ SA HP column to purify IgY from egg yolk solution.