Figure 2.

Isolation of IgY‐specific phage clones from T7 phage‐displayed random peptide libraries. (A) Isolation of IgY‐specific phage from the initial libraries (X₃CX_7‐8C₃ or X₃CX_9‐10C₃). After five rounds of biopanning, specific phage enrichment against IgY was examined by ELISA. Lib, phage libraries before biopanning; 1st–5th, phage after 1–5 rounds of biopanning; W, wild‐type phage; Np, measurement without phage. (B) Identification of IgY‐binding phage clones isolated from 4th and 5th phage pool by ELISA.