Figure 6.

Impact of five tomato AT‐Hook‐Like (AHL) proteins on immunity to Phytophthora capsici. Over‐expression of AHL1, AHL5 and AHL9 resulted in reduced P. capsici growth compared with an empty vector (EV) control. Virulence assays were performed in Nicotiana benthamiana plants over‐expressing enhanced green fluorescent protein (EGFP)‐AHLs. Plants were infiltrated with the Agrobacterium tumefaciens expression construct at OD 0.1. After 48 h, leaves were detached and 10‐μl drops of P. capsici spores (at a density of 50 000 ml⁻¹) were placed on the leaf surface. Lesion diameters were measured 48 and 72 h post‐infection (hpi). Significant difference relative to the EV control according to Dunnett's multiple comparison testing: *, P < 0.05. Error bars show ± SE.