Figure 11.

Proposed model for the light‐dependent activation of PiL[D24]. In the Dark state (left), PiL[D24] fluoresces (denoted by the green bars emanating out of the LOV2 domain). Light induces a conformational change in LOV2 that is transmitted (grey arrow on the right cartoon) to the flanking PKM2 part of the chimera, leading to an increase in its pyruvate kinase activity. Light activation leads to the loss of PiL[D24] fluorescence. C: cysteine in the LOV2 PAS domain; shown forming a cysteinyl adduct with FMN on the right.