Figure 3.

Cytokine secretion by circulating T cells in patients with PSC-UC compared with UC patients and controls. [A] Representative gating strategy for intracellular staining in PBMC. Cells were gated on CD45⁺ live cells, lymphocytes, and on CD3⁺, CD8⁺ or CD4⁺ cells. IFN-γ⁺, TNF-α⁺, IL-17A⁺, and IL-22⁺ cells were gated as shown. Gates were set using unstimulated conditions. [B] Frequency of IFN-γ⁺, TNF-α⁺, IL17-A⁺, and IL-22⁺ cells among CD3⁺, CD8⁺, and CD4⁺ cells in PBMC of patients with PSC-UC, UC and controls. †P < 0.05, Mann-Whitney test vs controls. PSC-UC, primary sclerosing cholangitis-ulcerative colitis; PBMC, peripheral blood mononuclear cells.