Table 1. . Primer sequences and PCR and Pyrosequencing® conditions.
| Gene | Forward primer | Reverse primer | PCR | Pyrosequencing | Ref. | ||||
|---|---|---|---|---|---|---|---|---|---|
| PCR Volume (μl) | Primer Concentration (ng) | Size (bp) | Annealing (°C) | Magnesium (μl) | Sequencing primer | ||||
| LINE1 | TTTTGAGTTAGGTGTGGGATATA | Biotin-AAAATCAAAAAATTCCCTTTC | 20 | 10 | 168 | 50 | 0 | AGTTAGGTGTGGGATATAGT | [16] |
| HOXA4 | TACACTTCACAAATTAATAACCATAAACTC | Biotin -GTTGTTGTAGYGGTAGGTGTTG | 25 | 75 | 188 | 63 | 1.5 | AACCCAAATTCCCTCCCTT | [17] |
| FKBP5 | ATTTGTAGTTGGGATAATAATTTGG | Biotin-AAATAAAATACAACTTTTCAAAACTAA | 25 | 75 | 240 | 58 | 1.5 | TGGAGTTATAGTGTAGGTTTT | |