Figure 1.
A Spindle-Independent Mechanism Shapes a Single Nucleus from a Set of Individualized Mitotic Chromosomes
(A) Live HeLa cells were imaged in the absence or presence of nocodazole and reversine. All cell lines used in this study stably express marker proteins as indicated (e.g., H2B-mCherry and Lap2β-EGFP here).
(B and C) Live cells were imaged (B), and the number of nuclei per post-mitotic cell was quantified (C) (bars indicate mean ± SD, n = 114 cells).
(D) Live HeLa cells as in (A) were imaged in the presence of taxol and reversine.
(E and F) Live cells as in were imaged (E), and the number of nuclei per post-mitotic cell was quantified (F) (bars indicate mean ± SD, n = 243). Scale bars, 10 μm.