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. 2017 Aug 24;170(5):956–972.e23. doi: 10.1016/j.cell.2017.07.038

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Figure S1 — Spindle Perturbations and Micronucleation Phenotypes, Related to Figure 1

(A–C) Live HeLa cells stably expressing H2B-mCherry and Tubulin-EGFP were imaged in (A) unperturbed condition, (B) in the presence of 200 ng/ml nocodazole or (C) in the presence of 500 nM taxol.

(D) Live cells stably expressing H2B–mCherry and the nuclear import substrate IBB-EGFP were imaged 20 hr after addition of 500 nM taxol and 320 nM reversine.

(E) Time-lapse images of live cells stably expressing H2B–mCherry and Lamin B-EGFP imaged in the presence of 500 nM taxol and 320 nM reversine. Some micronuclei that formed during mitotic exit under this condition collapsed over time (marked by yellow arrowheads).

Scale bars are 10 μm.