Figure S6.
BAF’s Interaction with Purified Chromatin In Vitro, Related to Figure 6
(A) Procedure for purification of chromatin from HeLa cells.
(B) Compositional analysis of whole cell extract and chromatin purified from this extract by Coomassie-stained SDS-PAGE gel. The profile plot refers to the purified chromatin lane. Core histones were annotated based on expected size and overlayed with immunoblot data against H2B.
(C) Immunoblotting of whole cell extract and chromatin purified from this extract using anti-H2B and anti-BAF antibodies, respectively.
(D) In vitro assay to probe interaction of recombinant BAF with purified chromatin. Time-lapse images of X-Z scans through the chromatin structure (box marks the cropped region of the chromatin structure that is shown in Figure 6A). BAF and VRK1 were added to the buffer as indicated, DNA was stained with Hoechst 33324.
(E) Time-lapse images of X-Z scans through the chromatin structure (box marks the cropped region of the chromatin structure that is shown in Figure 6D). BAFG47E was added to the buffer as indicated.
Scale bars are 10 μm.