Figure 4.
Inhibition of activated factor XII (α‐FXIIa) by recombinant corn trypsin inhibitor (rec‐CTI) variants and peptides. Concentrations of rec‐CTI variants (10−8 m to 10−5 m) or peptides (10−6 m to 10−3 m) were incubated with 200 μm substrate peptide, and this was followed by addition of α‐FXIIa; enzymatic activity was then monitored as described in Materials and methods. pIC 50 values were obtained by non‐linear regression (graphpad prism 6.04; log[inhibitor] versus response – variable slope algorithm with a bottom constraint). Error bars indicate the standard error (n = 3–5 independent observations).