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. 2017 Aug 25;10(10):761–771. doi: 10.1177/1756283X17725998

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© The Author(s), 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — P1-HNFα expression is decreased and P2-HNF4α expression is increased in HCC cell lines by polymerase chain reaction and western blotting (a–b). mRNA and protein levels of P1- and P2-HNF4α were measured in fresh HCC tissues (T) and corresponding adjacent nontumourous tissues (N) (c and e). Correlation of P1- and P2-HNF4α mRNA expression (r = −0.339, p = 0.023; d). Immortalized hepatocytes: L-02, MiHA; HCC cell lines: QGY-7703, HepG2, Huh-7, Bel-7402, QGY-7701, Hep3B, SMMC-7721, PLC, and SK-Hep-1.

HCC, hepatocellular carcinoma; HNF4α, hepatocyte nuclear factor 4 alpha; P1, promoter 1; P2, promoter 2.