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. 2017 Oct 12;12(10):e0184438. doi: 10.1371/journal.pone.0184438

Fig 5. Impact of LGG on C. albicans ergosterol content and glucose concentration in cell culture supernatants.

Fig 5

(A) Extracts of C. albicans grown on TR146 monolayers pretreated with either PBS or LGG were analyzed for their ergosterol content. (B) Exemplary selected ion chromatogram of one sample, where LGG-preincubated cells were infected with C. albicans, containing cholestane (m/z 217), cholesterol (m/z 368) from TR146 cells and ergosterol (m/z 363) from C. albicans. (C) Monolayers of TR146 cells were preincubated with PBS or LGG 12 h prior to infection with C. albicans cells. Amount of glucose was evaluated 6 h post infection. (D) Monolayers of TR146 cells were preincubated with PBS or LGG 12 h prior to infection. Directly before infecting epithelial cells with C. albicans, glucose was added to the experiment (5 mg/ml). LDH activity was quantified 6 h post infection. n = 3 *p<0.05, ***p<0.001.