Table 1. CFU of LGG and C. albicans used for different experiments.
Experiment | Format | Medium | No of TR146 cells | CFU of LGG |
CFU of C. albicans |
---|---|---|---|---|---|
Infection of RHOE | 6-well plate |
1 ml per well |
n/a | 3.8 x 106 per RHOE | 1 x 105 per RHOE |
Infection of monolayers | 24-well plate | 2 ml per well |
2 x 105 per well |
1.9 x 106 per ml | 5 x 105 per ml |
FUN-1 assay | 15 ml tube | 5 ml per tube |
n/a | 3.8 x 106 per ml | 2 x 106 per ml |
TEM analysis | 15 ml tube | 10 ml per tube |
n/a | 1.9 x 106 per ml | 1 x 106 per ml |
WST-1 assay | 15 ml tube / 96-well plate | 6 ml / 100 μl per tube / well |
n/a | 1.9 x 106 per ml | 5 x 105 per ml |
LGG growth | 15 ml tube | 8 ml per tube |
n/a | 1.9 x 106 per ml | n/a |
C. albicans growth | 15 ml tube | 8 ml per tube |
n/a | n/a | 1 x 106 per ml |
Adhesion | 96-well plate | 200 μl per well |
3 x 104 per well |
1.9 x 106 per ml | 5 x 105 per ml |
Invasion + hyphal growth | 24-well plate | 2 ml per well |
2 x 105 per well |
1.9 x 106 per ml | 5 x 105 per ml |
Numbers of human keratinocytes (TR146 cells) and colony forming units (CFU) of LGG and C. albicans used in the different experiments. Please note that adhesion assay equals one-tenth of monolayer and WST-1 assay one-twentieth, respectively.