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. 2017 Oct 12;12(10):e0184438. doi: 10.1371/journal.pone.0184438

Table 1. CFU of LGG and C. albicans used for different experiments.

Experiment Format Medium No of TR146 cells CFU of
LGG
CFU of
C. albicans
Infection of RHOE 6-well
plate
1 ml
per well
n/a 3.8 x 106 per RHOE 1 x 105
per RHOE
Infection of monolayers 24-well plate 2 ml
per well
2 x 105
per well
1.9 x 106 per ml 5 x 105
per ml
FUN-1 assay 15 ml tube 5 ml
per tube
n/a 3.8 x 106 per ml 2 x 106
per ml
TEM analysis 15 ml tube 10 ml
per tube
n/a 1.9 x 106 per ml 1 x 106
per ml
WST-1 assay 15 ml tube / 96-well plate 6 ml /
100 μl per tube / well
n/a 1.9 x 106 per ml 5 x 105
per ml
LGG growth 15 ml tube 8 ml
per tube
n/a 1.9 x 106 per ml n/a
C. albicans growth 15 ml tube 8 ml
per tube
n/a n/a 1 x 106
per ml
Adhesion 96-well plate 200 μl
per well
3 x 104
per well
1.9 x 106 per ml 5 x 105
per ml
Invasion + hyphal growth 24-well plate 2 ml
per well
2 x 105
per well
1.9 x 106 per ml 5 x 105
per ml

Numbers of human keratinocytes (TR146 cells) and colony forming units (CFU) of LGG and C. albicans used in the different experiments. Please note that adhesion assay equals one-tenth of monolayer and WST-1 assay one-twentieth, respectively.