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. 2017 Oct 12;12(10):e0186046. doi: 10.1371/journal.pone.0186046

Fig 1. IVIg inhibits anti-OVA IgG response in vivo in a dose-dependent manner but increases the weight of spleen and draining lymph nodes.

Fig 1

(A) Mouse immunization protocol. C57Bl/6NCrl mice were injected 3 times weekly subcutaneously in the neck with 50 μg Ovalbumin (Ova) and adjuvant AddaVax® (MF59), and terminated 24h after last injection. IVIg doses ranging from 1 mg to 50 mg were co-injected as indicated. (B) IgG mouse response against Ova measured by ELISA performed on mouse serum. Values are expressed as relative units to standard. Results pool data from 2 independent experiments (n = 6). Bars represent mean ± SD. n.d: not detected. Statistical significance was tested using one-way ANOVA (Dunnett’s test) where each group was compared to the control group ‘Adj + Ova’. (C) Pictures of spleen and draining lymph nodes (LN) from one representative animal in each group of treatment. (D) Weight of spleen and draining LN with number of B cells and CD4+ T cells as measured by flow cytometry. For weight, values are expressed as ratio to the ‘Adj + Ova’ treated group. For cell numbers, values are expressed as absolute cell numbers. Dead cells were excluded using a viability fluorescent dye. B cells were gated on CD19+ cells and T cells on CD4+ CD8- cells. Bars represent mean ± SD. Data pool 4 independent experiments (n = 12). Statistical significance was tested using one-way ANOVA (Dunnett’s test) where each group was compared to the control group ‘Adj + Ova’. Star maker significant difference *: p<0.05; **p<0 0.01; ***: p<0.001; ****: p<0.0001. n.d: not detected.