Fig 3. Inhibition experiments with PP PLA₂.

(A) Dose-response curve of PP PLA₂ inhibitory action on the ACh-evoked (25 μM ACh) ionic currents mediated by human α9α10 nAChR heterologously expressed in Xenopus oocytes. (B) Inhibition by PP PLA₂ of the initial rate of specific [¹²⁵I]-α-Bgt binding to T. californica and human hα7 nAChRs expessed in GH₄C₁ cells. Only 30% of binding sites in hα7 nAChRs could be protected from [¹²⁵I]-α-Bgt binding. (C) Inhibition by PP PLA₂ of specific [¹²⁵I]-α-Bgt binding to ECD of human α9 nAChR. IC₅₀ 5.5 μM.