Fig 4. Interaction of Cro with nAChR of T. californica electric organ, human neuronal α7 nAChR and AChBP.

(A) Inhibition of the initial rate of specific [¹²⁵I]-α-Bgt binding to T.californica nAChRs by Cro. Points were fit to a 2-site model with affinity for Cro of 30 nM and 260 nM. (B) Inhibition of the initial rate of specific [¹²⁵I]-α-Bgt binding to human α7 nAChRs by Cro. Two binding sites with affinity for Cro differing more than 3 orders of magnitude were revealed. (C) Inhibition of the initial rate of specific [¹²⁵I]-α-Bgt binding to acetylcholine-binding protein from L. staganlis by Cro.