Table 1. Summary of experimental design.

		GLYCOCALYX CONDITIONS
		Control	Degraded	Regenerated
		Untreated	Hep III	Self-Recovery (24 hrs after HepIII)	HS treated (16 hrs after HepIII)	S1P treated (16 hrs after HepIII)	HS/S1P treated (16 hrs after HepIII)
CULTURE CONDITIONS	DMEM + 1% P/S	x	x	x	x	x	x
	10% FBS	x	x	x	x	x	x
PRE-TREATMENT (2 hours)	DMEM + 1% P/S	x	x	x	x	x	x
	1% BSA	x	x	x	x	x	x
	2.5x10-6 IU Hep III		x	x	x	x	x
POST-TREATMENT	DMEM + 1% P/S	x	x	x	x	x	x
	1% BSA	x	x	x	x	x	x
	59 mg/ml HS				x		x
	10 μM S1P					x	x
ASSAYS OF EC FUNCTION	HS Expression	x	x	x	x	x	x
	Cx43 Expression at EC Borders	x	x	x	x	x	x
	Gap Junction Communication (Dye Transfer)	x	x	x	x	x	x

Abbreviations: Hep III is heparinase III; HS is heparan sulfate; S1P is sphingosine 1-phosphate; DMEM is Dulbecco’s Modified Eagle Medium; P/S is penicillin/streptomycin; FBS is fetal bovine serum; BSA is bovine serum albumin; IU is international units; Cx43 is connexin isoform 43.