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. 2017 Sep 8;16(20):1927–1932. doi: 10.1080/15384101.2017.1363941

Figure 1.

Figure 1.

Encapsulation of HAP stem-cell hair-spheres in PVDF-membrane cylinders for implantation to the severed sciatic nerve in nude and immunocompetent mice. HAP stem cells from the upper parts of vibrissa hair follicles from C57BL/6J mice were cultured in 10% FBS DMEM for 4 weeks. Growing HAP stem cells detached and were transferred to a non-adhesive culture dish in DMEM/F12 containing 2% B-27. After one week of culture, the detached cells formed hair spheres. Hair spheres were cultured on sterilized PVDF-membranes in 10% FBS DMEM for 3 d. PVDF-membranes was rolled up into cylinders with the hair spheres on the inside. Schematic diagram shows 2 transplantation designs. Experiment I: GFP-expressing HAP stem-cell hair-spheres from GFP-mouse hair follicles in PVDF-membrane cylinders were implanted into the severed sciatic nerve of nude mice (Crlj: CD1-Foxn1nu). Experiment II: A HAP stem-cell hair-sphere-containing PVDF-membrane cylinder from C57BL/6J mice were transplanted into the severed sciatic nerve of C57BL/6J mice.