Figure 3.
Effects of SURF6 overexpression on proliferation and viability of NIH/3T3-174 fibroblasts cultured without (-Dox) or with (+Dox) 100 ng/ml doxycycline up to 48 hours as analyzed with conventional phase contrast microscopy (A-F) and the MTT assay (G, H). (A-F) Cells were seeded in Petri dishes, cultured in complete growth medium for 3–4 hours to retrieve cell attachment to substrate. Twenty-five random fields of view were photographed shortly after doxycycline administration (“0” time-point, A) and 24 (B and C) and 48 (D and E) hours later, and representative images of the cells are shown in (A-E). Experiments were repeated five times, and in (F) bar graphs illustrating the mean cell number per field ± SEM are shown. The horizontal axis – time in hours, the vertical axis – the number of cells per square unit, small vertical bars – SEM. (G and H) Bar graphs illustrating MTT assay results. The horizontal axes – time in hours, the vertical axes in panel (G) – OD values at 570 nm, in panel (H) – the ODT /OD0 ratios equal to the OD values scored after 24 and 48 hours and normalized to the relative OD values at the “0” time-point. The data are presented as the mean ± SEM based on the results of three independent experiments.