Figure 2.

GFP^DIM adipose stromal cells have adipogenic capacity in vivo. GFP^DIM and GFP^NEG adipose stromal cells were purified from female wild type mice transplanted with BM from male mice constitutively expressing GFP by flow cytometric sorting as in Figure 1 (cells from n = 3–5 animals pooled/experiment). The cells were pelleted and resuspended in cold Matrigel at 2 × 10⁶ cells per 200 µl. The cell suspensions were injected subcutaneously in the abdomen anterior to the thigh of athymic female mice. Four weeks later the Matrigel plugs and adjacent skin and muscle were recovered, fixed in paraformaldehyde overnight and cut in half for embedding in paraffin. A) Five μm sections were deparaffinized and stained with Hematoxylin and Eosin. Figure shows images taken at 4x, 10x or 20x magnification of sections from mice receiving GFP^NEG or GFP^DIM cells. B) Additional sections were stained with a primary antibody to perilipin and Alexa 555-conjugated secondary antibody, and counterstained with DAPI. Fluorescence images taken at 40x show GFP and perilipin signals individually or overlaid with DAPI. Representative images of n = 3 independent experiments.