Figure 1.

To determine whether lipid droplets were present during osteoblast differentiation, neutral lipids were selectively stained with BODIPY493/503 (green puncta), while nuclei were stained with Hoechst (blue) in bone marrow stromal cells (BMSCs) under osteogenic conditions for 0 (A), 2 (B), or 7 (C) days; representative images are shown. To confirm osteogenic induction, relative mRNA expression of osteoblast-related genes runt-related transcript factor (Runx2) (D), osterix (Sp7) (E), and alkaline phosphatase (Alpl) (F) were determined in BMSCs through osteogenic differentiation (0, 2, or 7 days). Data shown is presented as mean ± standard error. Symbol, *, denotes statistically significant differences (P < 0.05) between groups.