Figure 2.
(A) Relative mRNA expression of lipid droplet-associated proteins from the PAT family of proteins including perilipin or Plin1 (Plin1), adipose differentiation-related protein or ADRP (Plin2), tail-differentiation interacting protein of 47 kDa or TIP47 (Plin 3), S3–12 (Plin4), and lipid storage droplet protein 5 or LSDP5 (Plin5) were detected in bone marrow stromal cells (BMSCs) under osteogenic conditions for 0, 2, or 7 days, femur cortex, and the bone marrow. All data are normalized to the housekeeping gene Hprt, and expressed relative to Plin1 expression in day 0 BMSCs. Data is represented as mean ± standard error. Uncorrected, mean CQ values are also indicated on graph for each target gene. (B) Plin2 protein abundance from BMSCs differentiated under osteogenic conditions for 0, 2, and 7 d. Mean protein abundance is expressed as density light units (DLUs x 103) relative to the loading control, β-actin.