Figure 3.

TR cells have EMT marker changes. (A) Western blotting analysis was used to detect the expression of E-cadherin, vimentin, ZEB1, N-cadherin, and Cancer Stem Cell marker, such as CD133 and Nestin in parental and TR glioma cells. (B) Quantitative results are illustrated for panel A. * P < 0.05; ** P < 0.01 vs their parental cells. (C) Real-time RT-PCR assay was conducted to detect the expression of miR-26b in parental and TR cells. * P < 0.05; ** P < 0.01 vs their parental cells. (D-E) Real-time RT-PCR was performed to detect the efficacy of miR-26b inhibitor and mimics transfection. * P < 0.05; *** P < 0.001 vs Control.