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. 2017 Oct 12;12(10):e0186187. doi: 10.1371/journal.pone.0186187

Fig 6. Bipartite nature of the Nab3 C-terminal essential region.

Fig 6

A) Yeast strains containing a plasmid bearing wildtype NAB3 on a URA3-marked plasmid and either the nab3Δ134α allele (strain DY353) or nab3Δ134αL800A allele (strain DY377) on LEU2-marked plasmids, were grown on complete medium (SC) or medium with FOA to test for viability after the loss of the former plasmid. Cells were diluted, spotted, and grown on a single plate of each indicated solid media at 30°C, along with a positive control (DY351; with wildtype NAB3 on a LEU2-marked plasmid) and negative control strain with a non-viable allele (nab3Δ191) of NAB3 (DY3183). B) The resulting shuffled FOA-resistant strains (DY359 [gray line] and DY379 [black line]) were tested for their termination competence as described in Materials and Methods. In lieu of reproducing the control strain peaks seen in Fig 3, only the position of the fluorescence maxima (arrows) for the termination-competent (+) (DY3217) and termination-defective (-) (DY3218) control strains are shown for reference.