Fig 5. Cardiac leiomodin2 binds to the sides of actin filaments.

(A) In high-speed cosedimentation assays F-actin (4 μM) and Lmod2^FL (0–5 μM) was incubated under high salt conditions (100 mM KCl, 2 mM MgCl₂) and centrifuged at 258.000 g for 30 min at room temperature. The pellets were analysed by SDS-PAGE. Representative Coomassie stained SDS-PAGE gel of the pellets is shown. (B) Lmod2^FL (5 μM) does not sediment in the absence of actin. Leiomodin was centrifuged in the absence of F-actin (left two columns) or F-actin (5 μM) was pelleted in the presence of Lmod2^FL (right two columns). Pellets and supernatants are indicated on the figure. (C) Actin-Lmod2^FL ratios in pellet were calculated from SDS-PAGE analysis of under high salt (HS, 100 mM KCl, 2 mM MgCl₂) and medium salt (MS, 50 mM KCl, 1 mM MgCl₂) conditions, and plotted as the function of Lmod2^FL concentration. Dashed lines show the fit using Eq 6. Data are presented as mean ± SD (n = 3).