Table 2. P. sungorus specific primer sequences used for standard plasmid generation and qPCR.

gene	5´3´sequence		melting	amplicon
			temperature	length
Agrp	forward	GCC TTT GCC CAA CAT CCG TTG	59.8	99 bp
	reverse	GCT ACT GCC GCT TCT TCA ATG CC	60.9
Bmal1	forward	GCT CAA GAG ACC CCA GGT TAT CC	59.1	145 bp
	reverse	GGC TCA TGA TGA CAG CCA TCG C	60.8
Dio2	forward	TGA AGA AAC ACA GGA GCC AAG AGG A	60.0	111 bp
	reverse	CAT TAT TGT CCA TGC GGT CAG CCA	59.8
Mct8	forward	GTC CTC TCA TTC CTG CTC CTG G	59.2	151 bp
	reverse	GTC CCA CCA GCT CAA ATG CAA TG	59.0
Npy	forward	CCA GGC AGA GAT ACG GCA AGA GAT C	60.7	119 bp
	reverse	CCA TCA CCA CAT GGA AGG GTC C	60.0
Per1	forward	CTC TTC TTC TGG CAA TGG CAA GGA C	60.0	120 bp
	reverse	GCA CTC AGG AGG CTA TAG GCA ATG	59.4
Actb	forward	ACC TCA TGA AGA TCC TGA CCG AGC	60.3	120 bp
	reverse	CCA TCT CTT GCT CGA AGT CCA GGG	61.1
Hprt	forward	AGT CCC AGC GTC GTG ATT AGT GAT G	60.4	140 bp
	reverse	CGA GCA AGT CTT TCA GTC CTG TCC A	60.5
Rplp0	forward	GCA ACA GTC GGG TAA CCA ATC TGC	60.4	153 bp
	reverse	CTTCGGGCTCATCATCCAGCAG	60.1
Rn18s	forward	GCT CCT CTC CTA CTT GGA TAA CTG TG	59.2	126 bp
	reverse	CGG GTT GGT TTT GAT CTG ATA AAT GCA	59.4