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. 2017 Oct 12;13(10):e1006650. doi: 10.1371/journal.ppat.1006650

Fig 5. Mutation PA D529N present in a virus from a fatal case reduces DVGs levels in virions of recombinant viruses with two different genetic backgrounds.

Fig 5

(A) DVGs proportion calculated as jumping reads/ reads per million (RPM) that align the viral genome, analyzed in purified virions from recombinant viruses generated on the A/H1N1/California/04/09 background. DVGs proportion of the CAL recombinant virus is taken as 1 and fold change is shown for mutant viruses versus CAL. CAL, wild type recombinant virus; PB2 mut, recombinant virus bearing PB2 221T mutation; PA mut, recombinant virus bearing PA 529N mutation; PB2/PA mut recombinant virus bearing PB2 221T and PA 529N (F-like-polymerase) mutations. (B) DVGs distribution per segment calculated as jumping RPM that align each viral segment, analyzed in purified virions from CAL, PB2 mut, PA mut and PB2/PA mut, M mut and M-PA mut recombinant viruses. (C) DVGs proportion as described in part A, CAL recombinant virus is taken as 1. M mut bearing M1 S30N and M2 V86S mutations; M-PA mut, recombinant virus bearing M1 S30N, M2 V86S and PA 529N mutations. (D) DVGs proportion, as described in (A) and (C), of all recombinant viruses. CAL is taken as 1 and fold change is shown for the other viruses. Viral segments, PB1, PB2, PA, HA, NP, NA, M, NS.