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. 2017 Oct 15;28(21):2757–2764. doi: 10.1091/mbc.E17-03-0206

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© 2017 Stout and Spray. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Cysteine residues in the CT of Cx32 restrict its mobility. (A) Three-dimensional time-lapse FRAP images of GFP tagged Cx32 and Cx32cyslCT in HeLa cells. The location of the border of the bleached region of the plaques is indicated by arrows; this border is not visible in the image of the msfGFP-Cx32cyslCT plaques 60 s postbleach because the unbleached protein has moved into the bleached region. (B) Percentage recovery of normalized fluorescence into the bleach region at 15 s for Cx32 and Cx32cyslessCT in HeLa cells using two-dimensional time-lapse FRAP. (C) The mobility of EBFP2-Cx30 (BCx30) is significantly reduced when it forms heterotypic GJ plaques with Cx32 compared with when it forms heterotypic gap junction plaques with Cx32cyslCT, as indicated by reduced recovery at 15 s for EBFP2-Cx30 at heterotypic plaques. N2A cells were used for experiments for C. Groups compared by Student’s t test (two-tailed).