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. 2017 Oct 15;28(21):2757–2764. doi: 10.1091/mbc.E17-03-0206

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© 2017 Stout and Spray. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Cellular redox balance controls channel arrangement within GJ plaques. (A) Three-dimensional time-lapse FRAP images of HeLa cells expressing msfGFP-Cx43 (untreated controls, top row), and three-dimensional time-lapse FRAP images of HeLa cells expressing msfGFP-Cx43, treated with 10 mM DTT for 6 h (bottom row). The location of the border of the bleached region of the plaques is indicated by arrows. (B) Percentage recovery of normalized fluorescence into the bleach region at 15 s for msfGFP-Cx43 in HeLa cells using two-dimensional time-lapse FRAP with and without 6-h treatment with 10 mM DTT. (C) Percentage recovery of normalized fluorescence into the bleach region at 15s for msfGFP-Cx32 with and without 6-h treatment with 10 mM DTT. Data for Cx43 and Cx32 are compared separately because there was a significant difference between sfGFP-Cx43 and sfGFP-Cx32 GJ length (Cx32 17.68 ± 2.926 µm vs. Cx43 11.85 ± 0.9938 µm, p = 0.0375). Data for untreated controls are sourced from previous figures of this report. Groups in B and C compared by Student’s t test (two-tailed).