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. 2017 Oct 15;28(21):2765–2772. doi: 10.1091/mbc.E17-05-0281

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© 2017 Chase et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — TorsinA forms homo-oligomeric ring structures in the presence of ATP that are disassembled by LAP1^LD or mutations of the “back” interface. (A) TorsinA (44 μM) was incubated with 2 mM of ATP or ADP and in the absence of nucleotide and the formation of complexes was assessed by SEC. Fractions were analyzed by negative stain electron microscopy. (B) Purified wild-type, G251D, and 3xD mutant TorsinA (44 μM each) were incubated in the presence of 2 mM ATP and complex formation assessed by SEC. (C) TorsinA (44 μM) was incubated with 2 mM ATP before the addition of 6:1 (mol:mol) Torsin:LAP1^LD (5 min. on ice) and complex formation assessed by SEC as before.